A Simple Key For thr77 Unveiled
A Simple Key For thr77 Unveiled
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which results in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant purpose in advertising mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two supplemental splice variants ended up reported but in-depth nucleotide sequence data isn't however out there.
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Blocking peptides are peptides that bind exclusively towards the goal antibody and block antibody binding. These peptide typically incorporates the epitope recognized with the antibody.
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This manual outlines additional possible optimization of cellular and immunoassay parameters to be sure the best possible final results are...
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to make certain the very best effects are...
Antibodies certain to the blocking peptide no longer bind into the epitope around the target protein. By comparing the staining through the blocked antibody versus the antibody alone, you can see which staining is particular.
Target Facts Cyclin E belongs on the very conserved cyclin loved ones, whose associates are characterized by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins functionality as regulators of CDK kinases. Different cyclins show distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic occasion. Cyclin E forms a posh with and features as being a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S changeover. This protein accumulates for the G1-S phase boundary and is particularly degraded as cells development through S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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